Research Article
A Novel Method for High-Level Production of TEV Protease by Superfolder GFP Tag
Table 2
Linker region of sfGFP-TEV-His6 Nd1–6.
| | Construction Code | Abbreviation | Linker length (aa) | Anticipated linker composition |
| | SfGFP-TEV-His6 Nd1 | Nd1 | 2 | THG/GS/RD | | SfGFP-TEV-His6 Nd2 | Nd2 | 5 | THG/GSKGP/RD | | SfGFP-TEV-His6 Nd3 | Nd3 | 8 | THG/NNPGSKGP/RD | | SfGFP-TEV-His6 Nd6 | Nd6 | 8 | THG/GSNLFKGP/RD | | SfGFP-TEV-His6 Nd4 | Nd4 | 11 | THG/NNPGSNLFKGP/RD | | SfGFP-TEV-His6 Nd5 | Nd5 | 14 | THG/MDPNNPGSNLFKGP/RD |
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