Methyl-CpG-Binding PCR of Bloodspots for Confirmation of Fragile X Syndrome in Males
(a) Relative locations of recognition sites of restriction enzymes MseI, MspI, and BstUI and MB-PCR primers. (b) Sequence of FMR1 promoter region. The methylated cytosine of CpG dinucleotides is indicated by an enlarged letter “C”; the recognition sites of MspI and BstUI are double underlined; the primers are indicated by arrows.
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