BioMed Research International / 2009 / Article / Fig 1

Methodology Report

Methyl-CpG-Binding PCR of Bloodspots for Confirmation of Fragile X Syndrome in Males

Figure 1

(a) Relative locations of recognition sites of restriction enzymes MseI, MspI, and BstUI and MB-PCR primers. (b) Sequence of FMR1 promoter region. The methylated cytosine of CpG dinucleotides is indicated by an enlarged letter “C”; the recognition sites of MspI and BstUI are double underlined; the primers are indicated by arrows.

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