BioMed Research International / 2009 / Article / Fig 4

Methodology Report

Methyl-CpG-Binding PCR of Bloodspots for Confirmation of Fragile X Syndrome in Males

Figure 4

Detection sensitivity of MB-PCR. (a) MB-PCR analysis using different amounts of input DNA, cycle numbers of the PCR amplification, and NaCl concentration in the stringency washes. (b) MB-PCR analysis of the DNA samples simulated as mosaic FXS by mixing 0.5 ng of DNA sample from an FXS male with varying amounts of DNA sample from a non-FXS male. (–): Control DNA with universally unmethylated sequences; (+): control DNA with universally methylated sequences; (M): molecular-size markers.

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