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Journal of Biomedicine and Biotechnology
Volume 2009, Article ID 643692, 8 pages
Methodology Report

Methyl-CpG-Binding PCR of Bloodspots for Confirmation of Fragile X Syndrome in Males

1Department of Pathology, Chi Mei Medical Center, Tainan, Taiwan
2Department of Pediatrics, Chi Mei Medical Center, Tainan, Taiwan
3The Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Kaohsiung, Taiwan
4Department of Pediatrics, Buddhist Tzu Chi General Hospital, Taipei Branch, Taipei, Taiwan

Received 28 February 2009; Revised 30 June 2009; Accepted 10 August 2009

Academic Editor: Wolfgang Schulz

Copyright © 2009 Ching-Cherng Tzeng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Expression and purification of the GST-tagged MBD2b and HIS-tagged MBD3L1 proteins were run on a 12.5% SDS-PAGE gel and stained with Coomassie blue. M, size marker; N, before IPTG induction; Lysate, cell lysates after induction.

  1. Supplementary Material