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Journal of Biomedicine and Biotechnology
Volume 2009, Article ID 646380, 8 pages
http://dx.doi.org/10.1155/2009/646380
Methodology Report

An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum

Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, Jiangsu 225009, China

Received 9 February 2009; Revised 28 April 2009; Accepted 26 May 2009

Academic Editor: Han De Winde

Copyright © 2009 Shi-Zhong Geng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

An asd-deleted ( asd) mutant of Salmonella enterica serovar Pullorum (SP) was constructed using an improved method of gene knockout by combining the -suicide plasmid system with the Red Disruption system. The asd gene was efficiently knocked out by the recombinant suicide vector, which replaced the asd gene with the gene. Based on the balanced lethal host-vector system, the phenotype of the asd mutant was further defined. The improved method was simpler and more effective than previously reported conventional methods.