BioMed Research International / 2009 / Article / Tab 1

Research Article

Epsilon Haemoglobin Specific Antibodies with Applications in Noninvasive Prenatal Diagnosis

Table 1

Specificity of the 384 selected antibody clones obtainted from the second round of selection against -Hb, tested in ELISA towards different antigens. E: recombinant denatured -Hb. RBC: parental red blood cell extract (containing - and -Hb, but no -Hb), BL21: bacterial control cell extract, and K562: K562 cell extract (containing -, -, -, -, and -Hb). Neg.: amount of clones not recognising any of the tested antigens. E RBC, E BL21, E K562, and E K562 BL21 RBC, amount of clones recognising -Hb (E) together with other antigens. A total of 178 (46%) of the tested clones ( ) gave positive ELISA signal toward -Hb, while 57 (15%) recognised K562 cell extract. Only two clones recognised K562 cell extract without recognising -Hb. Twelve of the selected antibodies showed specificity of the -Hb, since they recognised the denatured recombinant -Hb, the K562 extract, containing native -Hb and not the parental RBC, nor the BL21 extract.

ERBCBL21K562E RBCE BL21E K562E K562 BL21 RBCNeg.


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