Epsilon Haemoglobin Specific Antibodies with Applications in Noninvasive Prenatal Diagnosis
Specificity of the 384 selected antibody clones obtainted from the second round of selection against -Hb, tested in ELISA towards different antigens. E: recombinant denatured -Hb. RBC: parental red blood cell extract (containing - and -Hb, but no -Hb), BL21: bacterial control cell extract, and K562: K562 cell extract (containing -, -, -, -, and -Hb). Neg.: amount of clones not recognising any of the tested antigens. ERBC, EBL21, EK562, and EK562BL21RBC, amount of clones recognising -Hb (E) together with other antigens. A total of 178 (46%) of the tested clones () gave positive ELISA signal toward -Hb, while 57 (15%) recognised K562 cell extract. Only two clones recognised K562 cell extract without recognising -Hb. Twelve of the selected antibodies showed specificity of the -Hb, since they recognised the denatured recombinant -Hb, the K562 extract, containing native -Hb and not the parental RBC, nor the BL21 extract.
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