Production of Antibodies against Multipass Membrane Proteins Expressed in Human Tumor Cells Using Dendritic Cell Immunization
Figure 1
hSTEAP1 immunization. (a) Flow cytometry analysis to detect anti-hSTEAP1 in sera from immunized mice. Upper panels, DC-STEAP1 immunized (left), preimmunized (right). Lower panels, DC-empty immunized, with booster immunization (left), booster immunization only without DC immunization (right). 293T cells transiently transfected with pMRX-hSTEAP1-IRES-GFP were stained with sera, followed by PE conjugated antimouse IgG. (b) Western blotting analysis of hSTEAP1 immunization. The lysates of 293T cells and 293T cells transfected with pCAGGS-hSTEAP1 were probed with serum from immunized mice. (c) Anti-hSTEAP1 flow cytometry analysis in human prostate cancer cells. From left to right: LNCaP, DU145, and 293T cells. Solid line: anti-STEAP1 serum. Dashed line: preimmune serum.
