Production of Polyclonal Antibody against Interleukin-33 and Assessment of Its Distribution in Murine Liver and Lung
Figure 5
IL-33 immunostaining in mouse liver (a), (b), (e), (f) and lung (c), (d), (g), (h) tissues using the rabbit anti-IL-33 antibodies (1 : 1,000) under a microscope (400). Immunohistochemical analysis was performed in formalin-fixed, paraffin-embedded livers and lungs from normal mice and hydrodynamics-based gene injected mice after 24 hours (). Staining of IL-33 in livers and lungs from normal mice (b), (d) and hydrodynamics-injected mice (f), (h). Antibodies showed nuclear or cytoplasmic staining. Furthermore, there was a significant increase of IL-33 expression in hydrodynamics-injected mice (f), (h) compared with normal mice (b), (d). Control IgG showed no stain (a), (c), (e), (g).
