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Journal of Biomedicine and Biotechnology
Volume 2009 (2009), Article ID 736327, 8 pages
Research Article

A New Liquid Chromatography-Tandem Mass Spectrometry Method for Determination of Bisoprolol in Human Plasma Samples

1Center for Drug Evaluation, Antibiotice S.A., Valea Lupului, 1 Street, 707410 Iasi, Romania
2Department of Analytical Chemistry, Faculty of Pharmacy, “Gr.T. Popa” University of Medicine and Pharmacy, 700115 Iasi, Romania
3Faculty of Chemical Engineering and Environment Protection, Technical University “Gheorghe Asachi”, 71st A Mangeron Dimitrie Street, 7000050 Iasi, Romania

Received 6 March 2009; Revised 2 June 2009; Accepted 10 June 2009

Academic Editor: Kennerly S. Patrick

Copyright © 2009 Gabriela Peste et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Liquid chromatography (LC) coupled with mass spectrometry (MS) detection is one of the most powerful analytical tools for organic compound analysis. The advantages of using LC/MS methods over HPLC methods include: selectivity, chromatographic integrity, peak assignment, structural information, and rapid method development. In this paper, a new liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the determination of bisoprolol in human plasma samples, using metoprolol as internal standard and liquid-liquid extraction procedure. The assay has proven to be sensitive, specific and reproducible, suitable to determine the bisoprolol concentration, following a single oral administration of a 10 mg bisoprolol tablet in 22 healthy volunteers, in the bioequivalence study of Bisoprolol 10 mg coated tablets, produced by Antibiotice S.A. versus Concor 10 mg, produced by Merck.