A New Liquid Chromatography-Tandem Mass Spectrometry Method for Determination of Bisoprolol in Human Plasma Samples

Table 4

Stability of analyte following sample processing at room temperature.

	Analyte:		Bisoprolol
	Biological matrix:		Human Plasma
	Storage condition:		31 Hours at Room Temperature
	QC1 (3 ng/mL)			QC3 (75 ng/mL)
	Comparison Samples		Stability Samples	Comparison Samples		Stability Samples
	Measured	% nominal	Measured	Measured	% nominal	Measured
	Conc.		Conc.	Conc.		Conc.
	2.682	90.611	2.838	80.224	108.352	82.798
	2.330	78.719	2.639	82.755	111.771	63.262
	3.082	104.134	2.434	78.821	106.457	75.379
	2.380	80.392	2.079	79.826	107.815	80.616
	2.117	71.504	2.047	91.323	123.343	58.511
	2.619	88.475	2.745	84.105	113.595	79.410
N	6	6	6	6	6	6
Mean	2.535	85.639	2.464	82.842	111.889	73.329
SD (±)	0.338		0.338	4.597		10.049
CV(%)	13.314		13.734	5.549		13.704
% Change		−2.808			−11.483
	Acceptance criteria
	67% comparison samples must be 100±15% nominal values
	Mean % nominal of comparison samples 100±15%
	CV (%) ≤ 15%
	% Change ± 15%