A New Liquid Chromatography-Tandem Mass Spectrometry Method for Determination of Bisoprolol in Human Plasma Samples

Table 5

Stability of analyte in biological matrix at room temperature.

	Analyte:		Bisoprolol
	Biological matrix:		Human plasma
	Storage condition:		4 Hours at Room Temperature
	QC1 (3 ng/mL)			QC3 (75 ng/mL)
	Comparison Samples		Stability Samples	Comparison Samples		Stability Samples
	Measured	% nominal	Measured	Measured	% nominal	Measured
	Conc.		Conc.	Conc.		Conc.
	3.509	118.561	3.540	61.764	83.419	72.020
	2.839	95.898	3.502	74.312	100.368	67.462
	3.453	116.639	3.281	73.697	99.537	90.117
	3.187	107.684	3.514	72.186	97.495	73.715
	4.310	145.604	3.616	68.557	92.595	69.225
	3.123	105.510	3.688	75.832	102.421	69.012
N	6	6	6	6	6	6
Mean	3.403	114.983	3.523	71.058	95.973	73.592
SD (±)	0.506		0.138	5.182		8.405
CV(%)	14.864		3.925	7.292		11.421
% Change		3.522			3.566
	Acceptance criteria:
	67% comparison samples must be 100±15% nominal values
	Mean % nominal of comparison samples 100±15%
	CV (%) ≤15%
	% Change ± 15%