Expression and Purification of Active Recombinant Cathepsin C (Dipeptidyl Aminopeptidase I) of Kuruma Prawn <i>Marsupenaeus japonicus</i> in Insect Cells

<table>Immunoblot analysis on recombinant expression of CTSC in the culture media from 24 hours postinfected High Five cells by pIB/CTSC1  (with signal peptide, lane 1) and pIB/CTSC2 (without signal peptide, lane 2). The collected media from uninfected High Five cells was also loaded as a negative control (lane 3). Ten microliters of culture media in each lane was resolved on SDS-PAGE gel under reducing conditions and was electroblotted onto a PVDF membrane.  Recombinant CTSC fusion proteins were probed with anti-His-tag antibody.</table>

BioMed Research International

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Figure 2

Figure 2: Expression and Purification of Active Recombinant Cathepsin C (Dipeptidyl Aminopeptidase I) of Kuruma Prawn <i>Marsupenaeus japonicus</i> in Insect Cells 

Figure 2 | Expression and Purification of Active Recombinant Cathepsin C (Dipeptidyl Aminopeptidase I) of Kuruma Prawn Marsupenaeus japonicus in Insect Cells 