Expression and Purification of Active Recombinant Cathepsin C (Dipeptidyl Aminopeptidase I) of Kuruma Prawn Marsupenaeus japonicus in Insect Cells

Table 1

Purification and incubation of rCSTC secreted by insect cells.

	Total volume (mL)	Activity (U/mL)	Total activity (U)	Yield (%)
Concentrated culture medium	50	0.0036	0.18	100
Purified with Ni-chelating resin	5	0.0040	0.020	11.1
Incubated for 40 hours at pH5.5	5	0.0753	0.3765	209