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Journal of Biomedicine and Biotechnology
Volume 2009 (2009), Article ID 760540, 6 pages
Research Article

Silencing of Tumor Necrosis Factor Receptor 1 by siRNA in EC109 Cells Affects Cell Proliferation and Apoptosis

Department of Histology and Embryology, Medical College of Shantou University, Shantou, Guangdong Province 515041, China

Received 20 February 2009; Revised 16 July 2009; Accepted 23 July 2009

Academic Editor: Phillip Bird

Copyright © 2009 Ma Changhui et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Tumor necrosis factor receptor 1 (TNFR1) is a membrane receptor able to bind TNF- or TNF- . TNFR1 can suppress apoptosis by activating the NF- B or JNK/SAPK signal transduction pathway, or it can induce apoptosis through a series of caspase cascade reactions; the particular effect may depend on the cell line. In the present study, we first showed that TNFR1 is expressed at both the gene and protein levels in the esophageal carcinoma cell line EC109. Then, by applying a specific siRNA, we silenced the expression of TNFR1; this resulted in a significant time-dependent promotion of cell proliferation and downregulation of the apoptotic rate. These results suggest that TNFR1 is strongly expressed in the EC109 cell line and that it may play an apoptosis-mediating role, which may be suppressed by highly activated NF- B.