Table of Contents Author Guidelines Submit a Manuscript
Journal of Biomedicine and Biotechnology
Volume 2009, Article ID 767893, 8 pages
http://dx.doi.org/10.1155/2009/767893
Research Article

Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus

Department of Marine Biology, Ocean University of China, 5 Yushan Road, Qingdao 266003, China

Received 16 February 2009; Revised 1 June 2009; Accepted 18 June 2009

Academic Editor: Thomas Griffith

Copyright © 2009 Airong Jia and Xiao-Hua Zhang. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Chemokine receptor 4 (CXCR4) belongs to the large superfamily of G protein-coupled receptors. The EST sequence of CXCR4 from turbot (Scophthalmus maximus L.) was obtained from a subtractive cDNA library. In the present study, the full-length cDNA sequence of turbot CXCR4 was obtained, and sequence analysis indicated that its primary structure was highly similar to CXCR4 from other vertebrates. Quantitative real-time PCR demonstrated that the highest expression level of turbot CXCR4 was in the spleen following injection with physiological saline (PS). After turbot were challenged with Vibrio harveyi, the lowest expression level of CXCR4 was detected at 8 hours in the spleen and 12 hours in the head kidney, and then increased gradually to 36 hours. These findings suggested that CXCR4 may play a significant role in the immune response of turbot.