BioMed Research International / 2009 / Article / Fig 3

Research Article

In Vitro Ultramorphological Assessment of Apoptosis Induced by Zerumbone on (HeLa)

Figure 3

Fluorescent micrograph of acridine orange and propidium iodide double-stained human cervical cancer cells lines (HeLa). HeLa was treated at IC50 of ZER at time-dependent manner. Cells were cultured in RPMI 1640 media (25 mL flask) maintained at 37 and 5% CO2. (a) Untreated cells showed normal structure without prominent apoptosis and necrosis. (b) Early apoptosis features were seen after 24 hours representing intercalated acridine orange (bright green) amongst the fragmented DNA. (c) Blebbing and nuclear margination were noticed in 48-hour treatment of ZER. (d) Late apoptosis was seen in 72 hours incubated cells, whereby positive staining with orange color represents the hallmark of late apoptosis (magnification 400X).

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