BioMed Research International / 2009 / Article / Fig 6

Research Article

In Vitro Ultramorphological Assessment of Apoptosis Induced by Zerumbone on (HeLa)

Figure 6

(a) Electromicrograph of untreated human cervical cancer cells (HeLa) demonstrates the normal structure of HeLa cancer cell. Nucleus (N), nucleolus (NL), and the cytoplasm appeared without abnormal changes (X4600). (b) ZER-treated (24 hours) human cervical cancer cells (HeLa) demonstrate morphological features of early apoptosis: cell shrinkage, chromatin condensation (arrow), and integrity of plasma membrane (X6000). (c) ZER-treated human cervical cancer cells (HeLa) demonstrate the condensed cristae of mitochondria (MC) as a typical morphological feature in apoptosis (arrow) (X27500). (d) ZER-treated (48 hours) human cervical cancer cell line (HeLa) demonstrates morphological features of intermediate apoptosis: cell shrinkage, chromatin condensation (white arrow), and membrane blebbing (small white arrow) (X6000). (e) ZER-treated (72 hours) cervical cancer cells (HeLa) demonstrate morphological feature of late apoptosis: nuclear collapse, continuing blebbing, and apoptotic body formation (arrow) (X10 000). Cells were cultured in RPMI 1640 (25 mL flask) media maintained at 37 and 5% CO2.
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