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Journal of Biomedicine and Biotechnology
Volume 2009 (2009), Article ID 791432, 6 pages
Research Article

Protective Effect of Melatonin Against Mitomycin C-Induced Genotoxic Damage in Peripheral Blood of Rats

1Department of Pharmacology and Physiology, University of Zaragoza, 50009 Zaragoza, Spain
2Departments of Neurology and Medicine, Medical College of Wisconsin, Milwaakee, WI 53226, USA
3Department of Family and Community Medicine, Medical College of Wisconsin, Milwaakee, WI 53226, USA
4Department of Human Anatomy and Histology, University of Zaragoza, 50009 Zaragoza, Spain

Received 22 May 2009; Accepted 5 August 2009

Academic Editor: Gary S. Stein

Copyright © 2009 S. Ortega-Gutiérrez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Mitomycin C (MMC) generates free radicals when metabolized. We investigated the effect of melatonin against MMC-induced genotoxicity in polychromatic erythrocytes and MMC-induced lipid peroxidation in brain and liver homogenates. Rats ( = 36) were classified into 4 groups: control, melatonin, MMC, and MMC + melatonin. Melatonin and MMC doses of 10 mg/kg and 2 mg/kg, respectively, were injected intraperitoneally. Peripheral blood samples were collected at 0, 24, 48, 72, and 96 hours posttreatment and homogenates were obtained at 96 hours posttreatment. The number of micronucleated polychromatic erythrocytes (MN-PCE) per 1000 PCE was used as a genotoxic marker. Malondialdehyde (MDA) plus 4-hydroxyalkenal (4-HDA) levels were used as an index of lipid peroxidation. The MMC group showed a significant increase in MN-PCE at 24, 48, 72, and 96 hours that was significantly reduced with melatonin begin coadministrated. No significant differences were found in lipid peroxidation. Our results indicate that MMC-induced genotoxicity can be reduced by melatonin.