The Roles of Platelet GPIIb/IIIa and αvβ3 Integrins during HeLa Cells Adhesion, Migration, and Invasion to Monolayer Endothelium under Static and Dynamic Shear Flow
Figure 4
HeLa cell migration under shear flow in HeLa cells/HUVECs coculture parallel flow system. HeLa cells labelled by DiO were cultured onto the inner side of insert PET membrane, and the outer side seeded with HUVECs was exposed to 8 dyn/cm2 shear flow for 8 hours. The numbers of migrated HeLa cells were quantified by cell counting from more than six micrographic fields using magnification of 20×. (a) Flow and monolayer endothelium induced HeLa cells migration. * (EC+flow) versus (No EC+flow). (b) Migration of HeLa cells in the presence of thrombin-activated platelets (Pl+Th) or activated platelets with eptifibatide (Pl+Ep) under the above-mentioned flow condition. # versus control: ## (Pl+Ep) versus (Pl+Th). Control is considered as the sample of equal volume of cell culture medium added.
