Functional Expression of a DNA-Topoisomerase IB from Cryptosporidium parvum

Table 1

Sequences of the primers used in this study to clone and to mutate CpTopIB.

Specimen		Primers (5^′-3^′)
Wild type	FW	ATA AGA ATG CGG CCG CAT GGC ATC AGC TAC GGA TTC
	RV	GGA CTA GTT TAA AAT GAA AAA TCT GAT CTC G
R318A	FW	GAT TTT TTA GCT TTG GCG GTC GGA GGA GAA AAA G
	RV	C TTT TTC TCC TCC GAC CGC CAA AGC TAA AAA ATC
K358A	FW	CTT TAG ATT TTT TGG GCG CGG ATT CAA TTC GAT AC
	RV	GT ATC GAA TTG AAT CCG CGC CCA AAA AAT CTA AAG
R416A	FW	CT GCC AAG G TA TTT GCG ACA TTC AAT GCA TC
	RV	GA TGC ATT GAA TGT CGC AAA TAC CTT GGC AG
H489A	FW	GCG ATA TTA TGT AAC GCG CAG AGA TCA GTT CC
	RV	GG AAC TGA TCT CTG CGC GTT ACA TAA TAT CGC
Y600F	FW	CA TCG AAG ATT AAC TTT ATG GAT CCC AGG ATT TC
	RV	GA AAT CCT GGG ATC CAT AAA GTT AAT CTT CGA TG