Research Article
VHL Frameshift Mutation as Target of Nonsense-Mediated mRNA Decay in Drosophila melanogaster and Human HEK293 Cell Line
Table 1
Primer’s list used in this study.
| Name | Sequence - | Utilization |
| Upf1F | TTGGAATCATCACGCCTTACGA | Upf1 probe | Upf1R | CATGCCAACCGGAACTGGCATG | Upf1 probe | Upf1Fmut | ATGTCTTGCGTGTGTTCTAACGAACGT | Mutagenesis | Upf1Rmut | ACGTTCGTTAGAACACACGCAAGACAT | Mutagenesis | Vh | CCCTCAAGCCCTTCAGGAGGTGCGGGTGAAC | Vh mutagenesis | Vh | GGGAGTTCGGGAAGTCCTCCACGCCCACTTG | Vh mutagenesis | Vh | GGATGCACGTACGCTGCAGAGGATCTTTCA | Vh mutagenesis | Vh | TGAAAGATCCTCTGCAGCGTACGTGCATCC | Vh mutagenesis | Vh construct1(EcoRI) | CGCGCGGAATTCATGCCCCGGAGGGCGGAGAACT | Construct1 | VhR construct1(XhoI) | CGCGCGCTCGAGATGGTGAAACCCCGTCTCTACT | Construct1 | VhF1 construct1(XhoI) | CGCGCGCTCGAGTCAGGGGAAATGGAGAAAATAG | Construct1 | VhR1 construct1(XbaI) | CGCGCGTCTAGAGAGAATAGGATACAAAAAGATTGGA | Construct1 | c.163delGF | ACTGGGCGCCGAGGAGGAGATGAGGCCGGGCGGCCGC | Construct1 mutagenesis | c.163delGR | GCGGCCGCCCGGCCTCATCTCCTCCTCGGCGCCCAGT | Construct1 mutagenesis | VhF construct2(EcoRI) | CGCGCGGAATTCATGCCCCGGAGGGCGGAGAACT | Construct2 | VhR construct2(XhoI) | CGCGCGCCTCGAGTCAATCTCCCATCCGTTGATGTGC | Construct2 | c.172delGF | CAGGTCATCTTCTGCAATCGCATCCGCGCGTCGTGCT | Construct2 mutagenesis | c.172delGR | AGCACGACGCGCGGATGCGATTGCAGAAGATGACCTG | Construct2 mutagenesis | pVHLF | GGCCGCCGCATCCA | VHL QPCR | pVHL R | CATCGTGTGTCCCTGCATCTC | VHL QPCR | pGFP F | GCAACTACAAGACCCGC | GFP QPCR | pGFP R | GTCGGCCATGATATAGACG | GFP QPCR | rp49_F | CACACCGGAAACTCAATGGAT | rp49 QPCR | rp49_R | GGTCATCTTGAAGCTGGAAGG | rp49 QPCR | Act4A_F | GCTTCGCTGTCTACTTTCCA | Act4A QPCR | Act4A_R | CAGCCCGACTACTGCTTAGA | Act4A QPCR | Adh_RT_F | GGCGGTCCCGGTGGTA | RT-PCR, Adh QPCR | Adh_RT_R | CTGGTAGATGGCATTGAATCC | RT-PCR, Adh QPCR |
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