Bullatacin Triggered ABCB1-Overexpressing Cell Apoptosis via the Mitochondrial-Dependent Pathway
Effect of bullatacin on the release of cytochrome C from mitochondrial, activation of caspases (caspase-9, -8 and -3) and cleavage of PARP (a) and on the expression of bax, bcl-2 and Fas (b) in KBv200 cells. After incubation with bullatacin at desired concentrations for 48 h, the cellular proteins were subjected to western blot analysis. The experiments were performed three times and a representative experiment is shown; (c and d) Effect of bullatacin on capase-8 and -9 activation and apoptosis induction in KBv200 cells. KBv200 cells were incubated for 12 hours at C in the absence or presence of caspase inhibitors, at a concentration of 10 , prior to their exposure to 10 nmol/L bullatacin. 48 hours later, capase-8 and -9 activities were examined as the description of Section 2. Data represented means and standard errors of at least a triplicate determination.
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