Improved Coinfection with Amphotropic Pseudotyped Retroviral Vectors
Spinoculation increases infection of CRL-2703 human fibroblasts by amphotropic retroviral vectors produced by Plat-A cells. Cells were infected with an EGFP vector without spinoculation or with spinoculation at 600 g and 2400 g (in each case 2 hours at ). Representative microscope fields demonstrating increases in the fraction of infected cells (exhibiting green fluorescence) are shown.
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