Quantification of Spatial Parameters in 3D Cellular Constructs Using Graph Theory
Macro- and micro scale compaction occurs over 24 hours of 3.0 105 cell/mL gels. hMSCs at a cell density of 3.0 105 cells/mL were embedded in 2 mg/mL collagen I gels. Collagen I gels were fixed and stained for nuclei with SYTOX Green (a) Macroscale images of hydrogel compaction (Scale bar 1 cm). (b) Fluorescent image cross section of hMSC nuclei in collagen I gel (Scale bar 100 m).
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