Purification and Characterization of a Lectin from Phaseolus vulgaris cv. (Anasazi Beans)

Table 3

Effect of chemical modification on hemagglutinating activity of Anasazi bean lectin.

Treatment	Modified group/amino acid	% Hemagglutinating activity remaining
Phenylmethylsulfonyl fluoride (PMSF)	Serine	57
5, 5′-Dithiobis-(2-nitrobenzoic acid (DTNB)	Thiol group	100
Reductive methylation	Lysine	100
N-Bromosuccinimide (NBS)	Tryptophan	33
N-Acetylimidazole	Tyrosine	100