A New Caspase-8 Isoform Caspase-8s Increased Sensitivity to Apoptosis in Jurkat Cells
(a) Identification of a novel isoform of caspase-8 (caspase-8s): RT-PCR analysis of mRNA expression of caspase-8s from the bone marrow cells of AL individual. The PCR products were separated on 1.5% agarose gel and visualized by ethidium bromide staining. In addition to the expected bands (380 bp) representing wild type caspase-8, a small amplicon representing caspase-8s was identified (274 bp). (b) Coexpression of caspase-8 and caspase-8s in various cell lines: RT-PCR was carried out with RNA isolated from various cell lines as described under “Materials and Methods.” The PCR products were resolved on 1.5% agarose gel and visualized by ethidium bromide staining. GAPDH was used as a loading control. R = densitometrical units of caspase-8/densitometrical units of GAPDH.
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