A New Caspase-8 Isoform Caspase-8s Increased Sensitivity to Apoptosis in Jurkat Cells
Expression of Fas mRNA in empty vector or caspase-8s-transfected Jurkat cells and wild-type Jurkat cells was detected by semiquantitative RT-PCR: the PCR products were resolved on 1.5% agarose gel and visualized by ethidium bromide staining. The efficacy of RT-PCR was verified by detection of the human GAPDH mRNA. R = densitometrical units of Fas/densitometrical units of GAPDH.
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