BioMed Research International / 2009 / Article / Fig 11

Research Article

A New Caspase-8 Isoform Caspase-8s Increased Sensitivity to Apoptosis in Jurkat Cells

Figure 11

Expression of Fas Antigen in empty vector or caspase-8s–transfected Jurkat cells and wild-type Jurkat cells was analyzed by flow cytometry. (a) Analysed Jurkat cells were gated according to their size (FSC) and granularity (SSC). (b) Jurkat cell incubated with PE conjugated mouse IgG1 as isotype negative control. (c) JP4 (GFP positive cells) incubated with PE conjugated mouse IgG1 as isotype negative control. (d–f) Expression of Fas antigen in wild-type Jurkat cells, empty vector and, caspase-8s-transfected Jurkat cells. Numbers indicate the percentage of cells in the quadrant. Data shown are representative of three independent experiments.
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