A New Caspase-8 Isoform Caspase-8s Increased Sensitivity to Apoptosis in Jurkat Cells

Figure 3

SDS–PAGE analysis of caspase-8s protein expression in human AL bone marrow cells: lane 1–4: acute leukemia patient samples; 50 𝜇g of total protein from lysates of human AL bone marrow cells were subjected to 10% SDS-PAGE and immunoblotted with the rabbit antiprocaspase-8 corresponding to the N-terminal of human caspase-8 prodomain (C7849, Sigma). In addition to the two expected bands (55 kd or 53 kd) representing wild type caspase-8a/b, a small band (13 kd) representing caspase-8s was identified in lane 1.