A New Caspase-8 Isoform Caspase-8s Increased Sensitivity to Apoptosis in Jurkat Cells

Figure 5

Western bolt identified the protein expression of caspase-8s in caspase-8s-transfected cell clones: 50 𝜇g of total protein from lysates of empty vector (JP4 clone) or caspase-8s-transfected (JS2 clone, JS3 clone) Jurkat cells and wild-type Jurkat cells were subjected to 10% SDS-PAGE and immunoblotted with the rabbit antiprocaspase-8 (C7849, Sigma). A 13 kd caspase-8s protein was identified in JS2 and JS3. The 55 kd bands were endogenous caspase-8 protein.