BioMed Research International / 2009 / Article / Fig 6

Research Article

A New Caspase-8 Isoform Caspase-8s Increased Sensitivity to Apoptosis in Jurkat Cells

Figure 6

Caspase-8s promote cell apoptosis induced by Fas-agonistic antibody CH11: empty vector (JP4), caspase-8s-transfected (JS2, JS3) Jurkat cells and wild-type Jurkat cells were untreated or treated with 50 ng/mL and 75 ng/mL of CH11 for 24 h. After stimulation, the cells were collected and resuspended in standard buffer solution to a concentration of 106 cells/mL. Cells were incubated with in Annexin V-PE and 7-amino-actinomycin (7-AAD) for 15 min and analyzed on the FACScan. Early apoptotic cells were defined as those cells with Annexin V-PE staining in the absence of 7-AAD staining. Vector control transfected, caspase-8s-transfected, and wild type cells were analyzed in parallel. Apoptosis assays were run in triplicate revealing similar results (mean±SD).

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