Identification of the Leishmania major Proteins LmjF07.0430, LmjF07.0440, and LmjF27.2440 as Components of Fatty Acid Synthase II

Figure 4

Phenotype rescue of a yeast oar1Δ strain expressing LmjF27.2440. S. cerevisiae BY4741oar1Δ mutants producing native mitochondrial Oar1p, Cta1p, or mitochondrially targeted LmjF27.2440 were propagated on SD-Ura medium that applied selective pressure for plasmid maintenance, and following sequential tenfold dilution (triangle), the cells were spotted onto the indicated solid media. Respiration competence was examined by applying 2,3,5-triphenyltetrazolium chloride (TTC) as an overlay to the SD-Ura medium.