BioMed Research International / 2009 / Article / Tab 1

Research Article

Identification of the Leishmania major Proteins LmjF07.0430, LmjF07.0440, and LmjF27.2440 as Components of Fatty Acid Synthase II

Table 1

Plasmids and oligonucleotides used.

Plasmids or oligonucleotidesDescriptionSources or references

plasmid
1] pBluescript II KSpKS cloning vectorStratagene
pKS:LmjF07.0440 LmjF07.0440 in pBluescript (with primers for LmHTD2-1)This study
pKS:LmjF07.0430 LmjF07.0430 in pBluescript (with primers for LmHTD2-2)This study
pKS:LmjF27.2440 LmjF27.2440 in pBluescript (with primers for LmOAR1-1)This study
pKS:LmjF24.2030 LmjF24.2030 in pBluescript (with primers for LmOAR1-2)This study
2] YEp352URA3-marked multicopy plasmid[17]
3] YEp:CTA1 CTA1 behind its promoter (pYE352:CTA1)[18]
YEp:LmjF07.0440 LmjF07.0440 behind the CTA1 promoterThis study
YEp:LmjF07.0430 LmjF07.0430 behind the CTA1 promoterThis study
YEp:LmjF27.2440 LmjF27.2440 behind the CTA1 promoterThis study
YEp:LmjF24.2030 LmjF24.2030 behind the CTA1 promoterThis study
YEp:OAR1 OAR1 behind the CTA1 promoter[14]
YEp:YHR067w HTD2 behind the CTA1 promoter[12]
oligonucleotide
Lm HTD2-1 5 NcoITTATCCATGGAAGCGGCAGCACGCCGCATCATCThis study
Lm HTD2-1 3 HindIIITATTAAGCTTAAAGCCGACGCTCCACTCGCTCThis study
Lm HTD2-2 5 NcoITTATCCATGGCCAGCGAGACTGCACCACTTGTCThis study
Lm HTD2-2 3 HindIIITATTAAGCTTACTTCGTCACAGACCACTCACTCThis study
Lm OAR1-1 5 NcoITTATCCATGGGTGCCCCTAACGCACACCCACThis study
Lm OAR1-1 3 HindIIITATTAAGCTTACGATAAGCCGCCATCGACGGCGThis study
Lm OAR1-2 5 NcoITTATCCATGGACGCCAAGTCCATCCAGTCGThis study
Lm OAR1-2 3 HindIIITATTAAGCTTACTTGGCTGCCATACGGGTTGCCThis study

The numbers in superscript following the plasmids’ designation refer to their parental vectors, for example, pKS:LmjF07.0440 was derived from 1] pBluescript II KS.
Lm HTD2-1 refers to LmjF07.0440, Lm HTD2-2 to LmjF07.0430, Lm OAR1-1 to LmjF27.2440, and Lm OAR1-2 to LmjF24.2030.

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