BioMed Research International / 2009 / Article / Tab 1 / Research Article
Identification of the Leishmania major Proteins LmjF07.0430, LmjF07.0440, and LmjF27.2440 as Components of Fatty Acid Synthase II Table 1 Plasmids and oligonucleotides used.
Plasmids or oligonucleotides Description Sources or references plasmid
1] pBluescript II KS pKS cloning vector Stratagene pKS:LmjF07.0440
LmjF07.0440 in pBluescript (with primers for Lm HTD2-1) This study pKS:LmjF07.0430
LmjF07.0430 in pBluescript (with primers for Lm HTD2-2) This study pKS:LmjF27.2440
LmjF27.2440 in pBluescript (with primers for Lm OAR1-1) This study pKS:LmjF24.2030
LmjF24.2030 in pBluescript (with primers for Lm OAR1-2) This study 2] YEp352 URA3 -marked multicopy plasmid[17 ] 3] YEp:CTA1
CTA1 behind its promoter (pYE352:CTA1 )[18 ] YEp:LmjF07.0440
LmjF07.0440 behind the CTA1 promoter This study YEp:LmjF07.0430
LmjF07.0430 behind the CTA1 promoter This study YEp:LmjF27.2440
LmjF27.2440 behind the CTA1 promoter This study YEp:LmjF24.2030
LmjF24.2030 behind the CTA1 promoter This study YEp:OAR1
OAR1 behind the CTA1 promoter[14 ] YEp:YHR067w
HTD2 behind the CTA1 promoter[12 ] oligonucleotide
Lm HTD2-1 5
NcoI TTATCCATGGAAGCGGCAGCACGCCGCATCATC This study Lm HTD2-1 3
HindIII TATTAAGCTTAAAGCCGACGCTCCACTCGCTC This study Lm HTD2-2 5
NcoI TTATCCATGGCCAGCGAGACTGCACCACTTGTC This study Lm HTD2-2 3
HindIII TATTAAGCTTACTTCGTCACAGACCACTCACTC This study Lm OAR1-1 5
NcoI TTATCCATGGGTGCCCCTAACGCACACCCAC This study Lm OAR1-1 3
HindIII TATTAAGCTTACGATAAGCCGCCATCGACGGCG This study Lm OAR1-2 5
NcoI TTATCCATGGACGCCAAGTCCATCCAGTCG This study Lm OAR1-2 3
HindIII TATTAAGCTTACTTGGCTGCCATACGGGTTGCC This study
The numbers in superscript following the plasmids’ designation refer to their parental vectors, for example, pKS:LmjF07.0440
was derived from 1] pBluescript II KS.
Lm HTD2-1 refers to LmjF07.0440, Lm HTD2-2 to LmjF07.0430, Lm OAR1-1 to LmjF27.2440, and Lm OAR1-2 to LmjF24.2030.
