Novel miR390-Dependent Transacting siRNA Precursors in Plants Revealed by a PCR-Based Experimental Approach and Database Analysis

Figure 4

Nucleotide sequence alignment of “short” ta-siARF loci of cycad and conifer plants. The sequence of Cycas revoluta (undelined) was determined in this study, the others were taken from GeneBank. Boxes indicate miR390 complementary sites and ta-siARF sequence regions. Shading shows residues identical for the given position in most sequences. Sequences of C. revoluta miR390 complementary sites originate from primers used for the amplification of ta-siARF loci. GeneBank accession numbers of the sequences included in the alignment are as follows: Cycas revoluta, FJ804750; Picea sitchensis, EF086492; Pinus pinaster, BX682439; Pinus taeda, CV034496; Picea sitchensis, CO220369; Zamia fischeri, DY034932; Cryptomeria japonica, DC432705.