Impact of Lentiviral Vector-Mediated Transduction on the Tightness of a Polarized Model of Airway Epithelium and Effect of Cationic Polymer Polyethylenimine
Figure 4
Efficiency and effect on membrane permeability of PEI/LV vectors. Polarized 16HBE41o- cells were incubated with LV (50 MOI) either alone, formulated at different PEI molecules/TU ratios (A), or at different spermidine molecules/TU ratios (B) for 24 hours and then further incubated for 48 hours and evaluated for GFP expression. In another experimental setting, cells were pre-incubated with spermidine (range 0.08–8 mM) prior to addition of PEI/LV vector formulated at 1 × 107 ratio with 50 MOI LV (C). Note that PEI/LV increased the transduction efficiency by 3.6 fold as compared to plain LV. Data are expressed as means SD of two-three experiments. A: for 107 PEI/LV TU versus all the other conditions except 5 × 106. C: for all the conditions versus LV alone.
