Impact of Lentiviral Vector-Mediated Transduction on the Tightness of a Polarized Model of Airway Epithelium and Effect of Cationic Polymer Polyethylenimine
Figure 6
Cytotoxicity of PEI/LV vectors. Polarized 16HBE41o- cells were incubated with LV (50 MOI) either alone or formulated at different PEI molecules/TU ratios with 50 MOI LV for 24 hours and then analyzed for propidium iodide staining (a) or MTT assay (b). The same amounts of PEI used in the formation of PEI/LV vectors were tested for their cytotoxicity. Percentage of propidium iodide (PI)-positive cells incubated with medium only was 4.68 1.8 and subtracted from the other values. In the viability assay, untreated cells were incubated with medium only and considered as 100%. Data are expressed as means SD of two-three experiments. a: for 107 PEI/LV TU and free PEI versus all the other conditions except 5 × 106.
