An Improved Harvest and in Vitro Expansion Protocol for Murine Bone Marrow-Derived Mesenchymal Stem Cells
Figure 6
In vitro differentiation of mMSCs. The same amount of nonimmunodepleted mMSCs and ID-mMSCs were plated and adipogenic, osteogenic, and chondrogenic differentiation was evaluated at passage 3. Oil Red O staining ((a), ×200), von Kossa staining ((b), ×40 or ×100) and collagen type II staining ((c), ×100) show that ID-mMSCs exhibit much more differentiated cells, indicating a higher purity of multipotent MSCs with modified harvest method and immunodepletion. Representative pictures are shown from three independent experiments. *, scale bar = 100 μm.
