An Improved Harvest and in Vitro Expansion Protocol for Murine Bone Marrow-Derived Mesenchymal Stem Cells
Figure 7
Late passage ID-mMSCs retain tri-lineage differentiation potential. When exposed in induction medium, passage 8 ID-mMSCs can still exhibit differentiation potential into adipocytes, osteoblasts, and chondrocytes, as shown by Oil Red O staining ((a) ×200), von Kossa staining ((b) ×100) and collagen type II staining ((c) ×100). Representative pictures are shown from three independent experiments, scale bar = 100 μm.