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Journal of Biomedicine and Biotechnology
Volume 2010, Article ID 120328, 9 pages
Research Article

ICAM-1 Clustering on Endothelial Cells Recruits VCAM-1

Department of Molecular Cell Biology, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Plesmanlaan 125, 1066 CX Amsterdam, The Netherlands

Received 16 July 2009; Revised 12 November 2009; Accepted 20 December 2009

Academic Editor: Seamus J. Martin

Copyright © 2010 Jaap D. van Buul et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary figure 1: ICAM-1 and VCAM-1 both localize to apical protrusions. Anti-ICAM-1-antibody coated beads (diameter 10μm) were allowed to adhere to TNFα-treated HUVECs. Subsequently, the samples were fixed after 60 minutes and stained for ICAM-1 (green), VCAM-1 (red) and the merge in yellow. Actin is stained in white. Bar, 20μm. Lower panel shows X-Z projections of ICAM-1 in green and VCAM-1 in red protruding from the endothelial apical membrane, surrounding the bead.

  1. Supplementary Figure