BioMed Research International / 2010 / Article / Tab 4

Research Article

Epo Is Relevant Neither for Microvascular Formation Nor for the New Formation and Maintenance of Mice Skeletal Muscle Fibres in Both Normoxia and Hypoxia

Table 4

Indices of the microvascular supply and morphological data of gastrocnemius muscle fibers in normoxia (Nx), and after exposure to chronic hypoxia for 14 days (CHx) ( 𝑛 = 5 in each group).

NxCHx
Superficial regionDeep regionSuperficial regionDeep region
WTEpo-TA g h WTEpo-TA g h WTEpo-TA g h WTEpo-TA g h

Global microvessel indices
 CD (cap/mm²)546 ± 174611 ± 951699 ± 3501587 ± 180601 ± 105624 ± 811845 ± 3931701 ± 211
𝐶 / 𝐹 1.33 ± 0.161.70± 0.17*2.23 ± 0.292.69 ± 0.29*1.27 ± 0.151.65 ± 0.26*2.28 ± 0.202.62 ± 0.29*

Individual fiber microvessels indices
 CC2.89 ± 0.633.57 ± 0.56*5.20 ± 0.786.27 ± 0.54*2.95 ± 0.163.54 ± 0.46*5.22 ± 0.826.19 ± 0.41*
𝐶 / 𝐹 𝑖 1.05 ± 0.111.37 ± 0.21*2.16 ± 0.262.46 ± 0.35*1.09 ± 0.131.34 ± 0.20*2.15 ± 0.192.58 ± 0.21*
 LC/PF (%)8.96 ± 1.2611.9 ± 0.93*30.2 ± 7.335.4 ± 8.710.2 ± 1.8412.56 ± 0.82*32.5 ± 8.037.0 ± 3.90

Morphological data of muscle fibers
 FA ( 𝜇 m²)2521 ± 2862411 ± 1881259 ± 1581494 ± 1652189 ± 3142499 ± 2041232 ± 2461361 ± 133
 PF ( 𝜇 m)215 ± 17222 ± 21160 ± 16173 ± 14198 ± 13220 ± 7153 ± 18165 ± 11
 Form factor0.63 ± 0.020.63 ± 0.030.64 ± 0.060.64 ± 0.030.62 ± 0.020.63 ± 0.020.65 ± 0.040.65 ± 0.04

Values are means ± SD;
Main effect of Epo deficiency: significantly different from WT group: 𝑃 < . 0 5 .
CD: capillary density; 𝐶 / 𝐹 : capillary-to-fiber ratio; CC: number of capillaries around single fibers; 𝐶 / 𝐹 𝑖 : individual capillary-to-fiber ratio; LC/PF: length of the contact between microvessels and the fiber/perimeter of the fibers; FA: fiber area; PF: perimeter.

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