Optimization of the Fermentation Process of Actinomycete Strain Hhs.015T
Table 1
Effect of various carbon sources replacing starch and sucrose in SSY medium on antimicrobial activity of strain Hhs.01 to Candida vulgaris incubated 24 hours at on PDA medium.
Carbon sources
Diameter of inhibition zones (mm)
Price (RMB/Kg)
supplier
Contents of carbon %
Glucose
18.65 a
14
Fuchen Chemical Company, Tianjin
99.2
White sugar
18.33 a
4.5
Farmer’s Market, Yangling, Shaanxi
90.25
Brown sugar
17.86 ab
5
Farmer’s Market, Yangling, Shaanxi
80.32
Apple pomace (hydrolyzate)
17.45 ab
0.6
Juice factory, Qianxian, Shaanxi
15.08
Starch
16.21 ab
28
Fuchen Chemical Company, Tianjin
98.5
Control (Starch+ sucrose)
15.28 bc
29
Fuchen Chemical Company, Tianjin
98.5
Bran
13.15 c
1.2
Farmer’s Market, Yangling, Shaanxi
16.23
Black
7.78 d
0
Vinegar residue (hydrolyzate)
7.75 d
0.6
Farmer’s Market, Yangling, Shaanxi
10.15
Note: Inhibition zones with the same letter showed no significant difference analyzed by SAS statistics systems. The portion of carbon sources was about 1.5% (w/v). The control medium was the SSY medium. The Black had no starch and sucrose in SSY medium.