Optimization of the Fermentation Process of Actinomycete Strain Hhs.015T
Table 2
Effect of various nitrogen sources replacing soybean powder, yeast extract, and protease peptone in SSY medium on antimicrobial activity of strain Hhs.01 to Candida vulgaris incubated 24 hours at on PDA medium.
Nitrogen sources
Diameter of zones of inhibition (mm)
Price (RMB/Kg)
supplier
Contents of nitrogen
Rapeseed meal (hydrolyzate)
19.59 a
0.6
Farmer’s market, Yangling Shaanxi
1.67%
(NH4)2HPO4
17.32 ab
30
Fuchen Chemical Company, Tian Jin
20.31%
Cotton dregs (hydrolyzate)
17.20 ab
0.6
Farmer’s market, Yangling Shaanxi
3.1%
Control (soybean powder + yeast extract + protease peptone)
17.05 ab
245
Fuchen Chemical Company, Tian Jin
10%
(NH4)2SO4
16.75 ab
32
Fuchen Chemical Company, Tian Jin
17.92%
Fish meal (hydrolyzate)
16.91 ab
6
Farmer’s market, Yangling Shaanxi
3.33%
Black
16.49 ab
0
0
Urea
15.57 bc
2
Fuchen Chemical Company, Tian Jin
35.84%
NaNO3
13.11 bc
22
Fuchen Chemical Company, Tian Jin
17.92%
NH4NO3
0.00 d
28
Fuchen Chemical Company, Tian Jin
35.84%
Note: Inhibition zones with the same letter showed no significant difference analyzed by SAS statistics systems. 1.5% (w/v) of apple pomace was used as the carbon source replacing starch and sucrose in SSY medium except the control. The portion of nitrogen sources was about 0.7% (w/v). The control medium was the SSY medium. The Black: without soybean powder, yeast extract, and protease peptone in SSY medium
