Optimization of the Fermentation Process of Actinomycete Strain Hhs.015^T

Table 6

Analysis of the results of orthogonal design (L₁₆4⁵) for the optimization of strain Hhs.01 alternative cultural medium.

Runs					Diameter of zones of inhibition (mm)
1	1.5	0.1	0.06	0.01	16.0
2	1.5	0.2	0.07	0.02	15.6
3	1.5	0.3	0.08	0.03	14.2
4	1.5	0.4	0.09	0.04	15.0
5	2.0	0.1	0.08	0.04	13.2
6	2.0	0.2	0.09	0.03	14.8
7	2.0	0.3	0.06	0.02	12.6
8	2.0	0.4	0.07	0.01	15.9
9	2.5	0.1	0.09	0.02	9.2
10	2.5	0.2	0.08	0.01	9.9
11	2.5	0.3	0.07	0.04	9.2
12	2.5	0.4	0.06	0.03	15.1
13	3.0	0.1	0.07	0.03	7.4
14	3.0	0.2	0.06	0.04	16.0
15	3.0	0.3	0.09	0.01	14.1
16	3.0	0.4	0.08	0.02	9.9
	15.2	11.45	14.93	13.98
	14.16	14.08	12.03	11.83
	10.85	12.53	11.80	12.87
	11.85	13.98	13.28	13.35
	4.35	2.63	3.13	2.15

Note: Candida vulgaris was used as the target microbe inoculated 24 hours at on PDA medium in 20-cm-diameter Petri dish. One dish: one replication, the data in the table were the average of three replications.
, , , were apple pomace, rapeseed meal, MgSO₄7H₂O, and KH₂PO₄, respectively. is the average value of the level of one variable in line , and is the difference between the maximum and the minor average values of all of the levels of one variable in line , roughly explaining the effect of every variable on the antimicrobial activity
; ; ; ; .