BioMed Research International / 2010 / Article / Fig 5

Research Article

Insulin Promotes Survival of Amyloid-Beta Oligomers Neuroblastoma Damaged Cells via Caspase 9 Inhibition and Hsp70 Upregulation

Figure 5

Insulin enhances inducible Hsp70 activation triggered by rA 𝛽 42 oligomers. Western blot of proteins extracted from LAN5 untreated (1), treated with rA 𝛽 42 oligomers (40  πœ‡ M) for 1 h (2), rA 𝛽 42 oligomers (40  πœ‡ M) for 1 h and insulin (100  πœ‡ M) for 20 h (3), insulin alone (100  πœ‡ M ) for 20 h (4), incubated with anticonstitutive Hsp70 (Hsc70), antiinducible Hsp70 (Hsp70) or beta-actin ( 𝛽 -actin). Uniformity of gel loading was confirmed with 𝛽 -actin as standard. Quantification of immunoreactivity was performed using densitometric analysis.

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