Figure 1: The constitutive localization of the t-Akt1/PKB 𝛼 , phosphorylation of t-Akt1/PKB 𝛼 at Thr308 and at Ser473 in muscle fibers within muscle-tendon-bone unit. In comparison to the staining of t-Akt1/PKB 𝛼 (a, b), p-Akt1/PKB 𝛼 Thr308 (c, d) and Ser473 (e, f) in the peripheral muscle fibers, t-Akt1/PKB 𝛼 (b, MTJ), phosphorylation of Akt1/PKB 𝛼 at Thr308 (d, MTJ) and at Ser473 (f, MTJ) were detected with higher staining intensities in muscle fibers at the myotendinous junction (MTJ) area. The staining intensity of Akt1/PKB 𝛼 in peripheral muscle fibers (b, 602.70 ± 117.05) was weakly compared to those in the area near to the MTJ (b, 1050.63 ± 91.68). In comparison to the staining intensity of the p-Akt1/PKB 𝛼 Thr308 in peripheral muscle fibers (d, 319.76 ± 74.57), the staining intensity of p-Akt1/PKB 𝛼 Thr308 was higher in muscle fibers near to the MTJ areas (d, 1138.85 ± 98.99). The staining intensity of p-Akt1/PKB 𝛼 Ser473 in muscle fibers at the MTJ areas (f, 351.65 ± 104.63) was greater than staining intensity of Ser473 in peripheral muscle areas (f, 1099.70 ± 135.93). Data are mean ± SD; 𝑛 = 6; significant differences were considered at a 𝑃 value < . 0 5 . MTJ = myotendinous junction, pM = peripheral muscle fibers, ab = alveolar bone, pdl = periodontal ligament, p = pulpa, d = dentin. Bars: 640  𝜇 m for (a), (c), (e), 80  𝜇 m for (b), (d), and (f).