BioMed Research International / 2010 / Article / Fig 3

Research Article

Bone Marrow Derivation of Interstitial Cells of Cajal in Small Intestine Following Intestinal Injury

Figure 3

Whole mount preparation of small intestine from both normal C57 mouse and chimeric mouse. in this figure, donor’s BMDCs were detected by GFP antibody. Macrophages were demonstrated by their specific marker F4/80. Upper panel: C57 mouse was used as normal control, in which all the macrophages were negative for GFP antibody. Bar = 5 0 𝜇 m . Lower panel: in the chimerism some macrophages were double positive for GFP and F4/80, suggesting that they were derived from the infused donor’s BMDCs. GFP+/F4/80+ cells were mainly found at the deep myenteric plexus and they were scattered in this layer, which distinguished them from ICCs. Bar = 5 0 𝜇 m .
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