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Journal of Biomedicine and Biotechnology
Volume 2010, Article ID 168689, 7 pages
http://dx.doi.org/10.1155/2010/168689
Research Article

Upregulation of Endogenous HMOX1 Expression by a Computer-Designed Artificial Transcription Factor

1Department of Anatomy, Third Military Medical University, Gao-Tan-Yan Street, Sha-Ping-Ba District, Chongqing 400038, China
2Department of Immunology, Third Military Medical University, Gao-Tan-Yan Street, Sha-Ping-Ba District, Chongqing 400038, China

Received 1 March 2010; Revised 25 May 2010; Accepted 17 June 2010

Academic Editor: Kostas Iatrou

Copyright © 2010 Hongfeng Guo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Heme oxygenase-1 (HO-1) is well known as a cytoprotective factor. Research has revealed that it is a promising therapeutic target for cardiovascular diseases. In the current study, an HMOX1 (HO-1 gene) enhancer-specific artificial zinc-finger protein (AZP) was designed using bioinformatical methods. Then, an artificial transcription factor (ATF) was constructed based on the AZP. In the ATF, the p65 functional domain was used as the effector domain (ED), and a nuclear localization sequence (NLS) was also included. We next analyzed the affinity of the ATF to the HMOX1 enhancer and the effect of the ATF on endogenous HMOX1 expression. The results suggest that the ATF could effectively upregulate endogenous HMOX1 expression in ECV304 cells. With further research, the ATF could be developed as a potential drug for cardiovascular diseases.