Review Article

Dielectrophoresis: A Review of Applications for Stem Cell Research

Figure 2

Theoretical modelling of the polarisability factor ๐‘ of (1) and (3), as a function of frequency, for a viable cell suspended in a solution of conductivity 40โ€‰mS/m. The modelling employs the double-shell representation of a nucleated cell [13]. The plot is normalized against the polarisability of a conducting sphere of the same diameter. With increasing frequency, the membrane capacitance electrically shorts out the membrane resistance, allowing the applied field to penetrate through the cytoplasmic membrane, and the cellโ€™s polarisability approaches that ( ๐‘ = 1) of a conducting sphere. Changes in cell diameter, from 10โ€‰ ๐œ‡ m to 20โ€‰ ๐œ‡ m, alter the polarisability for frequencies below โˆผ 1โ€‰MHz.