Figure 5: Distribution of vimentin-positive cells (left column) and of nestin-positive cells (right column) in the renal interstitium of control (a, b) or at different time intervals after ischemia-reperfusion (c–h). In control (a), vimentin-positive fusiform cells are scarce within interstitium (arrows) of OSOM. Focally in S3 tubules, undifferentiated epithelial cells devoid of brush border (arrowhead) appeared positive. Two and three days after reperfusion (c, e) vimentin-positive fusiform cells are localized around S3 necrotic tubules (NT) in OSOM. At the same period (2 days—(g)), their density is higher within ISOM. (b) Distribution of nestin positive cells in interstitium of OSOM in a control animal. Two days after reperfusion (d), nestin-positive fusiform cells form a dense network around necrotic tubules (NT) in OSOM. Focally, isolated epithelial cells appeared positive in altered tubules (d inset). Later, 7 days after reperfusion (f), positive cells are restricted to periphery of cystic tubules (CT). In ISOM (h), the density of fusiform positive cells is particularly high 2 days after reperfusion. Scale bar. 1 8 𝜇 m : (a, c); 2 5 𝜇 m : (d); 3 0 𝜇 m : (b, e, f, g, h).