Figure 8: (a) Double label immunofluorescence for nestin (green) and vimentin (red) in an ischemic kidney two days after reperfusion. Interstitial cells coexpressing both markers appear yellow (arrows). (b) Double immunostaining for vimentin (red) and PCNA nuclei (green) two days after reperfusion. PCNA-positive nuclei were observed in interstitial fusiform vimentin-positive cells. (c) Double label immunofluorescence for 𝛼 -SMA (red) and nestin (green) in ischemic kidney one week after reperfusion. Most of interstitial cells surrounding cystic tubules coexpressed both markers (yellow). Scale bar. 1 8 𝜇 m : (a, b, c).